Background: Persistent infection with high-risk Human
Papilloma Virus (HPV) causes development of cervical
cancer. Among the high risk group, HPV-16 accounts for
50% & HPV-18 accounts for 12% of cervical cancer.
Objective: The current sudy aimed to evaluate the prevalence
of HPV genotype 16 and 18 in high grade cervical
intraepithelial neoplasia (CINII&III) and cervical cancer.
Methods: This descriptive type of cross-sectional observational
study was carried out in colposcopy clinic of Mymensingh
Medical College Hospital (MMCH), Bangladesh, from July
2012 to June 2013. Women with colposcopically diagnosed
high-grade CIN and clinically diagnosed cervical carcinoma
were enrolled. Pregnant & menstruating women were
excluded from this study. Colposcopy guided cervical biopsy
were taken from high grade CIN with loop electrosugical
excision procedure (LEEP) and wedge biopsy were taken
from cervical cancer. All the specimen were reviewed by
histopathologist to confirm the diagnosis. Extra sample of
specimen were sent to the Department of Microbiology and
Hygiene of Bangladesh Agriculture University, Mymensingh
for the detection of HPV-16 & 18 DNA by Polymerase Chain
Reaction (PCR).

Result: Out of 71 samples, histopathologically 4.2% was
diagnosed as chronic cervicitis, 8.5% CIN I, 26.8% CIN
II, 16.9% CIN III, 40.8% squamous cell carcinoma and
2.8% adenocarcinoma of cervix. The results revealed that
30 cases were positive for HPV-16, 06 cases for HPV-18
and 04 cases for both. In this study, the prevalence of HPV-
16 & 18 infection was 56.3%. Infection found to be higher
in women aged between 45 and 54 (P<0.01). Most of them
were from low socioeconomic status (P<0.01) and married
at an early age ranging from 11 to 15 (P<0.01). It was
found that multiparity correlated higher rate of HPV
positivity than only one or two pregnancy, but statistically it
was not significant (P>0.05).
Conclusion: The high prevalence of HPV-16 and 18 in high
grade CIN and cervical cancer samples suggests that
vaccination against HPV-16 and 18 may be effective in
bringing down the cervical cancer incidence in Bangladesh.
Key Words: Prevalence, HPV-16 & 18, High-Grade
Cervical Intraepithelial Neoplasia, Cervical Cancer.
(J Bangladesh Coll Phys Surg 2018; 36: 112-117)
DOI: http://dx.doi.org/10.3329/jbcps.v36i2.36079